When cells had detached, adding of preheated (37 °C) standard cell culture medium II, inhibited the digesting enzyme trypsin. Suspensions were pipetted into. 50
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The organic phase was carefully transferred into a 2 mL vial for GC–MS analysis. dichloromethane from 5 mL samples with deuterated internal standards.
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Operating and maintenance costs have been kept to a min mum. Unitrex can process 10 samples simultaneously, and only small amounts of solvent and gas are
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