May 15, 2013 · Each vial was labeled and a light layer of fluorinated grease (Dupont TM Krytox® LVP, Sigma-Aldrich) was applied to the lip of the vial to aid in sealing. The empty 2 mL vial was tared on the balance, approximately 1 mL of liquid was added, and the mass of liquid added, m L 0 , was determined by reweighing the vial plus liquid.

The tube just thawed is re-frozen. The ether phase is decanted into respective, labeled glass scintillation vials. The vials are left in the fume hood to evaporate the ether overnight. Alternatively, the ether can be evaporated under N2 to shorten the evaporation time. Day 2: 14. 15. When the scintillation vials are dry, 1 ml of phosgel buffer

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